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MF-Human C5a(Complement Component 5a) ELISA Kit

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Product Name	MF-Human C5a(Complement Component 5a) ELISA Kit	Species	Human
Uniprot ID	P01031	Alternative Names	C5a
Detection method	Sandwich	Sensitivity	46.88 pg/mL
Standard	5000pg/mL	Detection Range	78.13-5000pg/mL
Sample type	Serum, Plasma and Tissue homogenate and Other biological samples；Sample volume：25μL
Reaction time	3H	Research Area	Signaling transduction, Cell biology, Cancer, Immunology, Metabolism;
Test principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C5a. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Human C5a. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C5a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human C5a. You can calculate the concentration of Human C5a in the samples by comparing the OD of the samples to the standard curve.