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MF-Human GROα/CXCL1(Growth Regulated Oncogene Alpha) ELISA Kit

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Product Name	MF-Human GROα/CXCL1(Growth Regulated Oncogene Alpha) ELISA Kit	Species	Human
Uniprot ID	P09341	Alternative Names	NAP3, GRO1, GRO-A, MGSA, MGSA-A, SCYB1, FSP, CINC-1, Platelet-derived growth factor-inducible protein KC, Secretory protein N51
Detection method	Sandwich	Sensitivity	9.38 pg/mL
Standard	1000pg/mL	Detection Range	15.63-1000pg/mL
Sample type	Serum, Plasma and Tissue homogenate and Other biological samples；Sample volume：25μL
Reaction time	3H	Research Area	Cancer, Immunology;
Test principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GROα/CXCL1. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Human GROα/CXCL1. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GROα/CXCL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human GROα/CXCL1. You can calculate the concentration of Human GROα/CXCL1 in the samples by comparing the OD of the samples to the standard curve.