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MF-Human IFN-α(Interferon Alpha) ELISA Kit

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Product Name	MF-Human IFN-α(Interferon Alpha) ELISA Kit	Species	Human
Uniprot ID	P01562	Alternative Names	IFNα, IFNA,IFN-A,INFA,INF-A,INF-α, INFα
Detection method	Sandwich	Sensitivity	2.34 pg/mL
Standard	250pg/mL	Detection Range	3.90-250pg/mL
Sample type	Serum, Plasma and Tissue homogenate and Other biological samples；Sample volume：25μL
Reaction time	3H	Research Area	Cardiovascular, Cell biology, Neuroscience, Cancer, Immunology, Metabolism
Test principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IFN-α. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Human IFN-α. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IFN-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human IFN-α. You can calculate the concentration of Human IFN-α in the samples by comparing the OD of the samples to the standard curve.