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MF-Human IL-8(Interleukin 8) ELISA Kit

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Product Name MF-Human IL-8(Interleukin 8) ELISA Kit Species Human
Uniprot ID P10145 Alternative Names 3-10C, AMCF-I, C-X-C motif chemokine 8, CXCL8, CXCL8SCYB8, Emoctakin, GCP1, GCP-1TSG-1, IL8, IL-8, interleukin 8, K60, LAI, LECT, LUCT, LYNAP, MDNCF, MDNCFb-ENAP, member 8, MONAP, MONAPGCP1, NAF, NAP1, NAP-1NAP1, NCF, Neutrophil-activating protein 1, Protein 3-10C, T cell chemotactic factor, T-cell chemotactic factor, TCF, TSG1
Detection method Sandwich Sensitivity 0.56 pg/mL
Standard 200pg/mL Detection Range 3.12-200pg/mL
Sample type Serum, Plasma and Tissue homogenate and Other biological samples;Sample volume:25μL
Reaction time 3H Research Area Tumor immunity;
Test principle This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-22. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Human IL-22. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-22, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human IL-22. You can calculate the concentration of Human IL-22 in the samples by comparing the OD of the samples to the standard curve.

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