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MF-Human LEP(Leptin) ELISA Kit

Product Name	MF-Human LEP(Leptin) ELISA Kit	Species	Human
Uniprot ID	P41159	Alternative Names	LEPD, OBS, Obesity Homolog
Detection method	Sandwich	Sensitivity	9.38 pg/mL
Standard	1000pg/mL	Detection Range	15.63-1000pg/mL
Sample type	Serum, Plasma and Tissue homogenate and Other biological samples；Sample volume：25μL
Reaction time	3H	Research Area	Neuroscience, Signaling transduction, Cancer;
Test principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LEP. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Human LEP. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LEP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human LEP. You can calculate the concentration of Human LEP in the samples by comparing the OD of the samples to the standard curve.