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MF-Mouse GROα/CXCL1(Growth Regulated Oncogene Alpha) ELISA Kit

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Product Name MF-Mouse GROα/CXCL1(Growth Regulated Oncogene Alpha) ELISA Kit Species Mouse
Uniprot ID P12850 Alternative Names NAP3, GRO1, GRO-A, MGSA, MGSA-A, SCYB1, FSP, CINC-1,KC/CXCL1,keratinocyte-derived Cytokine,KC,N51,IL-8 functional homologue
Detection method Sandwich Sensitivity 9.38 pg/mL
Standard 1000pg/mL Detection Range 15.63-1000pg/mL
Sample type Serum, Plasma and Tissue homogenate and Other biological samples;Sample volume:25μL
Reaction time 3H Research Area Cardiovascular, Cell biology, Neuroscience, Cancer, Immunology, Metabolism
Test principle This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GROα/CXCL1. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Mouse GROα/CXCL1. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GROα/CXCL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Mouse GROα/CXCL1. You can calculate the concentration of Mouse GROα/CXCL1 in the samples by comparing the OD of the samples to the standard curve.

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