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MF-Rat MCP-1(Monocyte Chemotactic Protein 1) ELISA Kit

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Product Name	MF-Rat MCP-1(Monocyte Chemotactic Protein 1) ELISA Kit	Species	Rat
Uniprot ID	P14844	Alternative Names	CCL2, GDCF-2, HC11, HSMCR3, MCAF, MCP1, SCYA2, SMC-CF
Detection method	Sandwich	Sensitivity	0.1 ng/mL
Standard	10ng/mL	Detection Range	0.16-10ng/mL
Sample type	Serum, Plasma and Tissue homogenate and Other biological samples；Sample volume：25μL
Reaction time	3H	Research Area	Cell biology, Cancer, Immunology;
Test principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MCP-1. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Rat MCP-1. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MCP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat MCP-1. You can calculate the concentration of Rat MCP-1 in the samples by comparing the OD of the samples to the standard curve.