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MF-Rat VEGF-A(Vascular Endothelial Cell Growth Factor A) ELISA Kit

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Product Name	MF-Rat VEGF-A(Vascular Endothelial Cell Growth Factor A) ELISA Kit	Species	Rat
Uniprot ID	P16612	Alternative Names	VEGF-A, VEGFA, MVCD1, VPF
Detection method	Sandwich	Sensitivity	18.75 pg/mL
Standard	2000pg/mL	Detection Range	31.25-2000pg/mL
Sample type	Serum, Plasma and Tissue homogenate and Other biological samples；Sample volume：25μL
Reaction time	3H	Research Area	Signaling transduction, Cell biology, Cancer, Immunology, Metabolism ;
Test principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat VEGF-A. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Rat VEGF-A. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat VEGF-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat VEGF-A. You can calculate the concentration of Rat VEGF-A in the samples by comparing the OD of the samples to the standard curve.