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MF-Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

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Product Name	MF-Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit	Species	Mouse
Uniprot ID	P06804	Alternative Names	DIF, TNF-alpha, TNFA, TNFSF2, TNF-A, TNFα
Detection method	Sandwich	Sensitivity	6.56 pg/mL
Standard	700pg/mL	Detection Range	10.94-700pg/mL
Sample type	Serum, Plasma and Tissue homogenate and Other biological samples；Sample volume：25μL
Reaction time	3H	Research Area	Cardiovascular, Cell biology, Neuroscience, Cancer, Immunology, Metabolism
Test principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNF-α. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Mouse TNF-α. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Mouse TNF-α. You can calculate the concentration of Mouse TNF-α in the samples by comparing the OD of the samples to the standard curve.