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Megazyme® α-Amylase (Bacillus licheniformis)

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High purity α-Amylase (Bacillus licheniformis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. For use in Megazyme Dietary Fiber methods, suitable for use at pH 6.5 and above. E-BLAAM-A-100mL specifically to be used with ANKOMTDF Dietary Fiber Analyzer.
   
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Specifications
Brand Megazyme®
Approvals EBC Method 6.5
EC Number 3.2.1.1
Storage Conditions Storage Temperature: 2-8°C Stability: > 4 years at 4°C
Application Examples For use in Megazyme Dietary Fiber methods.
Formulation Stabilised solution
Physical Form Solution
CAS Number 9000-90-2, 9000-85-5
Synonyms alpha-amylase; 4-alpha-D-glucan glucanohydrolase
Source Bacillus licheniformis
Molecular Weight 58000
Expression Purified from Bacillus licheniformis
Specificity endo-hydrolysis of α-1,4-D-glucosidic linkages in starch.
Specific Activity ~ 55 U/mg (40°C, pH 6.5 on Ceralpha reagent)
Unit Definition One Unit of α-amylase is the amount of enzyme required to release one µmole of p-nitrophenol from blocked p-nitrophenyl-maltoheptaoside per minute (in the presence of excess α-glucosidase) at pH 6.0 and 40°C.
Temperature Optima (°C) 75
Package Dimensions 1.20 in x 1.20 in x 3.70 in
Package Weight 0.18 lb
   

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