The Enterobacteriaceae vial is a screening vial specific for Enterobacteriaceae organisms and can be used for specification monitoring in food and nutraceutical products. The vial has broad inclusivity and an assay time of 14-24 hours for most applications. The vial contains a peptone yeast extract base with glucose as the carbon source. The selective agents include bile salts, sodium lauryl sulfate and other Gram-positive inhibitors. Acidification of the medium due to glucose utilization changes the pH. As Enterobacteriaceae organisms metabolize, the pH indicator changes from purple to a yellow color.